↑Teodora Dan was appointed as "Miss Universe Romania 2016" after a casting call was held by the national director Ernest Hadrian Böhm, who obtained the franchise license of Miss Universe in Romania.[9]
↑Darina Kulsitova was appointed to compete at Miss Universe 2016 by the Miss Kazakhstan Organization after the original winner of Miss Kazakhstan 2015, Regina Valter was disqualified after getting married. The announcement was made a day before the contestants would officially start arriving in the Philippines. Darina previously competed at the Miss Kazakhstan 2014 national pageant.
↑Jenny Kim was appointed to compete at Miss Universe 2016 by Park Jeong-ah, the national director of the Miss Universe Korea pageant and also the new franchise holder for Miss Universe in Korea. Kim replaces Min-ji Lee, Miss Korea 2015, who would not compete at the Miss Universe 2016 pageant after Hanju E&M, the organizers of the Miss Korea pageant, lost the Miss Universe franchise. Coincidentally, Park Jeong-ah also holds the franchise for Miss World in Korea, and Kim was the 1st runner-up at the Miss World Korea 2015 pageant.[ต้องการอ้างอิง]
↑Kushboo Ramnawaj was handpicked to represent Mauritius at Miss Universe 2016 by Nevin Rupear, the national director of the Estrella Mauritius Organisation, after he was appointed the new franchise holder for Miss Universe in Mauritius. Ramnawaj replaces Danika Atchia, Miss Mauritius 2015, who was crowned by the previous franchise holder Primerose Obeegadoo and would have represented Mauritius at Miss Universe 2016 otherwise. Ramnawaj was apparently crowned Miss Mauritius 2014 and was supposed to compete at the Miss Universe 2015 pageant but was replaced because of a disagreement with the former franchise holder.[7]
↑Brenda Jimenez was crowned as the new Miss Universe Puerto Rico 2016 by Desiree Lowry, the national director of the Miss Universe Puerto Rico pageant, after Kristhielee Caride, the original winner, was dethroned due to her dismissive behaviour.[8] Later, Caride challenged her dethronement in court, which ruled against her. Jimenez represented the municipality of Aguadilla at Miss Universe Puerto Rico 2016 and was the 1st runner-up at the pageant.[ต้องการอ้างอิง]
↑Yuliana Korolkova was appointed Miss Universe Russia 2016 by the Miss Russia Organization as a replacement to Yana Dobrovolskaya, the winner of the Miss Russia 2016 pageant, who will compete only at Miss World 2016 due to the conflicting schedules of the two international pageants. The winner of the Miss Russia pageant usually participates at both Miss Universe and Miss World pageants. Korolkova represented Orenburg Oblast at Miss Russia 2016 and was the 1st runner-up at the pageant.[10]