English: Summed slices z-projection of actin filaments in mouse cortical neurons grown in vitro for 2.5 days. Image was taken on a zeiss 780 confocal microscope then deconvoluted using an experimental point spread function.

Scan Settings Pinhole [m]: 53.0 µm Total Size-Width: 168.65 µm Total Size-Height: 168.65 µm Total Size-Depth: 5.50 µm StepSize: 0.125 µm Voxel-Width: 41.0 nm (pixel size) Voxel-Height: 41.0 nm (pixel size) Voxel-Depth: 125.0 nm Zoom: 0.8 Objective: Plan-Apochromat 63x/1.40 Oil DIC

Numerical aperture: 1.40